Our latest paper on vaporization of intact neutral biomolecules using laser-based thermal desorption is out now in J. Am. Soc. Mass. Spec. – Well done Yerbolat and Siwen! https://doi.org/10.1021/jasms.3c00194
Recent News
Poster at FEMTO15
Grite is off to the FEMTO15 conference in Berlin, presenting our work on using UV-XUV pump-probe spectroscopy to follow roaming dynamics in acetaldehyde. You can also find the poster in our gallery.
New paper in Molecules
Our latest paper on High-Throughput UV Photofragmentation is out now in Molecules – well done Siwen and Yerbolat! https://doi.org/10.3390/molecules28135058
New paper in JASMS
Our latest paper on vaporization of intact neutral biomolecules using laser-based thermal desorption is out now in J. Am. Soc. Mass. Spec. – Well done Yerbolat and Siwen! https://doi.org/10.1021/jasms.3c00194
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Recent Publications
Warne, Emily M.; Smith, Adam D.; Horke, Daniel A.; Springate, Emma; Jones, Alfred J. H.; Cacho, Cephise; Chapman, Richard T.; Minns, Russell S.
Time Resolved Detection of the S(1D) Product of the UV Induced Dissociation of CS2 Journal Article
In: J. Chem. Phys., vol. 154, no. 3, pp. 034302, 2021, ISSN: 0021-9606, 1089-7690.
@article{Warne:J.Chem.Phys.154:034302,
title = {Time Resolved Detection of the S(1D) Product of the UV Induced Dissociation of CS2},
author = {Emily M. Warne and Adam D. Smith and Daniel A. Horke and Emma Springate and Alfred J. H. Jones and Cephise Cacho and Richard T. Chapman and Russell S. Minns},
url = {http://aip.scitation.org/doi/10.1063/5.0035045},
doi = {10.1063/5.0035045},
issn = {0021-9606, 1089-7690},
year = {2021},
date = {2021-01-01},
urldate = {2021-06-21},
journal = {J. Chem. Phys.},
volume = {154},
number = {3},
pages = {034302},
abstract = {The products formed following the photodissociation of UV (200 nm) excited CS2 are monitored in a time resolved photoelectron spectroscopy experiment using femtosecond XUV (21.5 eV) photons. By spectrally resolving the electrons, we identify separate photoelectron bands related to the CS2 + h$nu$ textrightarrow S(1D) + CS and CS2 + h$nu$ textrightarrow S(3P) + CS dissociation channels, which show different appearance and rise times. The measurements show that there is no delay in the appearance of the S(1D) product contrary to the results of Horio et al. [J. Chem. Phys. 147, 013932 (2017)]. Analysis of the photoelectron yield associated with the atomic products allows us to obtain a S(3P)/S(1D) branching ratio and the rate constants associated with dissociation and intersystem crossing rather than the effective lifetime observed through the measurement of excited state populations alone.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The products formed following the photodissociation of UV (200 nm) excited CS2 are monitored in a time resolved photoelectron spectroscopy experiment using femtosecond XUV (21.5 eV) photons. By spectrally resolving the electrons, we identify separate photoelectron bands related to the CS2 + h$nu$ textrightarrow S(1D) + CS and CS2 + h$nu$ textrightarrow S(3P) + CS dissociation channels, which show different appearance and rise times. The measurements show that there is no delay in the appearance of the S(1D) product contrary to the results of Horio et al. [J. Chem. Phys. 147, 013932 (2017)]. Analysis of the photoelectron yield associated with the atomic products allows us to obtain a S(3P)/S(1D) branching ratio and the rate constants associated with dissociation and intersystem crossing rather than the effective lifetime observed through the measurement of excited state populations alone.
Sobolev, Egor; Zolotarev, Sergei; Giewekemeyer, Klaus; Bielecki, Johan; Okamoto, Kenta; Reddy, Hemanth K. N.; Andreasson, Jakob; Ayyer, Kartik; Barak, Imrich; Bari, Sadia; Barty, Anton; Bean, Richard; Bobkov, Sergey; Chapman, Henry N.; Chojnowski, Grzegorz; Daurer, Benedikt J.; Dörner, Katerina; Ekeberg, Tomas; Flückiger, Leonie; Galzitskaya, Oxana; Gelisio, Luca; Hauf, Steffen; Hogue, Brenda G.; Horke, Daniel A.; Hosseinizadeh, Ahmad; Ilyin, Vyacheslav; Jung, Chulho; Kim, Chan; Kim, Yoonhee; Kirian, Richard A.; Kirkwood, Henry; Kulyk, Olena; Küpper, Jochen; Letrun, Romain; Loh, N. Duane; Lorenzen, Kristina; Messerschmidt, Marc; Mühlig, Kerstin; Ourmazd, Abbas; Raab, Natascha; Rode, Andrei V.; Rose, Max; Round, Adam; Sato, Takushi; Schubert, Robin; Schwander, Peter; Sellberg, Jonas A.; Sikorski, Marcin; Silenzi, Alessandro; Song, Changyong; Spence, John C. H.; Stern, Stephan; Sztuk-Dambietz, Jolanta; Teslyuk, Anthon; Timneanu, Nicusor; Trebbin, Martin; Uetrecht, Charlotte; Weinhausen, Britta; Williams, Garth J.; Xavier, P. Lourdu; Xu, Chen; Vartanyants, Ivan A.; Lamzin, Victor S.; Mancuso, Adrian; Maia, Filipe R. N. C.
Megahertz Single-Particle Imaging at the European XFEL Journal Article
In: Commun Phys, vol. 3, no. 1, pp. 97, 2020, ISSN: 2399-3650.
@article{Sobolev:CommunPhys3:97,
title = {Megahertz Single-Particle Imaging at the European XFEL},
author = {Egor Sobolev and Sergei Zolotarev and Klaus Giewekemeyer and Johan Bielecki and Kenta Okamoto and Hemanth K. N. Reddy and Jakob Andreasson and Kartik Ayyer and Imrich Barak and Sadia Bari and Anton Barty and Richard Bean and Sergey Bobkov and Henry N. Chapman and Grzegorz Chojnowski and Benedikt J. Daurer and Katerina D\"{o}rner and Tomas Ekeberg and Leonie Fl\"{u}ckiger and Oxana Galzitskaya and Luca Gelisio and Steffen Hauf and Brenda G. Hogue and Daniel A. Horke and Ahmad Hosseinizadeh and Vyacheslav Ilyin and Chulho Jung and Chan Kim and Yoonhee Kim and Richard A. Kirian and Henry Kirkwood and Olena Kulyk and Jochen K\"{u}pper and Romain Letrun and N. Duane Loh and Kristina Lorenzen and Marc Messerschmidt and Kerstin M\"{u}hlig and Abbas Ourmazd and Natascha Raab and Andrei V. Rode and Max Rose and Adam Round and Takushi Sato and Robin Schubert and Peter Schwander and Jonas A. Sellberg and Marcin Sikorski and Alessandro Silenzi and Changyong Song and John C. H. Spence and Stephan Stern and Jolanta Sztuk-Dambietz and Anthon Teslyuk and Nicusor Timneanu and Martin Trebbin and Charlotte Uetrecht and Britta Weinhausen and Garth J. Williams and P. Lourdu Xavier and Chen Xu and Ivan A. Vartanyants and Victor S. Lamzin and Adrian Mancuso and Filipe R. N. C. Maia},
url = {http://www.nature.com/articles/s42005-020-0362-y},
doi = {10.1038/s42005-020-0362-y},
issn = {2399-3650},
year = {2020},
date = {2020-12-01},
urldate = {2021-06-21},
journal = {Commun Phys},
volume = {3},
number = {1},
pages = {97},
abstract = {Abstract The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement of significantly more experimental data per day than was previously possible. The European XFEL is expected to provide 27,000 pulses per second, over two orders of magnitude more than any other XFEL. The increased pulse rate is a key enabling factor for single-particle X-ray diffractive imaging, which relies on averaging the weak diffraction signal from single biological particles. Taking full advantage of this new capability requires that all experimental steps, from sample preparation and delivery to the acquisition of diffraction patterns, are compatible with the increased pulse repetition rate. Here, we show that single-particle imaging can be performed using X-ray pulses at megahertz repetition rates. The results obtained pave the way towards exploiting high repetition-rate X-ray free-electron lasers for single-particle imaging at their full repetition rate.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Abstract The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement of significantly more experimental data per day than was previously possible. The European XFEL is expected to provide 27,000 pulses per second, over two orders of magnitude more than any other XFEL. The increased pulse rate is a key enabling factor for single-particle X-ray diffractive imaging, which relies on averaging the weak diffraction signal from single biological particles. Taking full advantage of this new capability requires that all experimental steps, from sample preparation and delivery to the acquisition of diffraction patterns, are compatible with the increased pulse repetition rate. Here, we show that single-particle imaging can be performed using X-ray pulses at megahertz repetition rates. The results obtained pave the way towards exploiting high repetition-rate X-ray free-electron lasers for single-particle imaging at their full repetition rate.
Samanta, Amit K.; Amin, Muhamed; Estillore, Armando D.; Roth, Nils; Worbs, Lena; Horke, Daniel A.; Küpper, Jochen
Controlled Beams of Shock-Frozen, Isolated, Biological and Artificial Nanoparticles Journal Article
In: Structural Dynamics, vol. 7, no. 2, pp. 024304, 2020.
@article{Samanta:StructuralDynamics7:024304,
title = {Controlled Beams of Shock-Frozen, Isolated, Biological and Artificial Nanoparticles},
author = {Amit K. Samanta and Muhamed Amin and Armando D. Estillore and Nils Roth and Lena Worbs and Daniel A. Horke and Jochen K\"{u}pper},
url = {https://aca.scitation.org/doi/10.1063/4.0000004},
doi = {10.1063/4.0000004},
year = {2020},
date = {2020-03-01},
urldate = {2020-07-21},
journal = {Structural Dynamics},
volume = {7},
number = {2},
pages = {024304},
publisher = {American Institute of Physics},
abstract = {X-ray free-electron lasers promise diffractive imaging of single molecules and nanoparticles with atomic spatial resolution. This relies on the averaging of millions of diffraction patterns of identical particles, which should ideally be isolated in the gas phase and preserved in their native structure. Here, we demonstrated that polystyrene nanospheres and Cydia pomonella granulovirus can be transferred into the gas phase, isolated, and very quickly shock-frozen, i.e., cooled to 4,K within microseconds in a helium-buffer-gas cell, much faster than state-of-the-art approaches. Nanoparticle beams emerging from the cell were characterized using particle-localization microscopy with light-sheet illumination, which allowed for the full reconstruction of the particle beams, focused to $<$100$mu$m$<$100,$mu$m$<$math display="inline" overflow="scroll" altimg="eq-00001.gif"$>$ $<$mrow$>$ $<$mo$>\&$lt;$<$/mo$>$ $<$mn$>$100$<$/mn$>$ $<$mo$>$,$<$/mo$>$ $<$mi$>mu<$/mi$>$ $<$mi mathvariant="normal"$>$m$<$/mi$><$/mrow$><$/math$>$, as well as for the determination of particle flux and number density. The experimental results were quantitatively reproduced and rationalized through particle-trajectory simulations. We propose an optimized setup with cooling rates for particles of few-nanometers on nanosecond timescales. The produced beams of shock-frozen isolated nanoparticles provide a breakthrough in sample delivery, e.g., for diffractive imaging and microscopy or low-temperature nanoscience.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
X-ray free-electron lasers promise diffractive imaging of single molecules and nanoparticles with atomic spatial resolution. This relies on the averaging of millions of diffraction patterns of identical particles, which should ideally be isolated in the gas phase and preserved in their native structure. Here, we demonstrated that polystyrene nanospheres and Cydia pomonella granulovirus can be transferred into the gas phase, isolated, and very quickly shock-frozen, i.e., cooled to 4,K within microseconds in a helium-buffer-gas cell, much faster than state-of-the-art approaches. Nanoparticle beams emerging from the cell were characterized using particle-localization microscopy with light-sheet illumination, which allowed for the full reconstruction of the particle beams, focused to $<$100$mu$m$<$100,$mu$m$<$math display="inline" overflow="scroll" altimg="eq-00001.gif"$>$ $<$mrow$>$ $<$mo$>&$lt;$<$/mo$>$ $<$mn$>$100$<$/mn$>$ $<$mo$>$,$<$/mo$>$ $<$mi$>mu<$/mi$>$ $<$mi mathvariant="normal"$>$m$<$/mi$><$/mrow$><$/math$>$, as well as for the determination of particle flux and number density. The experimental results were quantitatively reproduced and rationalized through particle-trajectory simulations. We propose an optimized setup with cooling rates for particles of few-nanometers on nanosecond timescales. The produced beams of shock-frozen isolated nanoparticles provide a breakthrough in sample delivery, e.g., for diffractive imaging and microscopy or low-temperature nanoscience.